We provide customized solutions for your image analysis problems. Below lists some of the application and software that we have developed for our collaboration projects. In addition, we also provide trainings in various microscopy image analysis software such as ImageJ, Imaris, Amira, Matlab etc.

Please feel free to contact us for any request regarding any of the software listed on this page.

Applications

 Cell segmentation

Various segmentations from single cell segmentation for filopodia measurement, to multi cellular segmentation for cell-cell junction analysis.

(Source : https://www.biorxiv.org/content/10.1101/195420v2, image courtesy of Dr Naila Alieva)

 

 Geometry analysis

Geometry analysis of epithelial gap and cell colony for studying relations of geometry to cell migration.

Curvature analysis for epithelial gap closure.

(Source : https://www.nature.com/articles/ncomms8683, image courtesy of Dr Andrea Ravasio)

 

 Motion analysis

Motion analysis for collective cell migration, cortical flow, cytoplasmic streaming.

Quantification of rotational and expanding motion for cell colony expansion

(Source : https://pubmed.ncbi.nlm.nih.gov/26402903/, image courtesy of Dr Andrea Ravasio)

motion_input_output.png
 

 Fiber segmentation

Fiber segmentation and measurements of length, orientation, tortuosity for actin cables, radial fibers, myosin stacks.

(Source : https://www.molbiolcell.org/doi/full/10.1091/mbc.E19-02-0082 , image courtesy of Dr Hu Shiqiong)

 

Software

 Imaris

Imaris is a software developed by Oxford Instruments for 3D and 4D visualization, measurement and tracking.

(Source : image courtesy of Dr Wong Bin Sheng)

 

 Huygens

Huygens is a software specialized in deconvolution and microscopy image restoration.

Example of deconvolution using Huygens.

(Source : image courtesy of Dr Selwin Wu Kaixiang)

 

 Fiji

Fiji is an image processing package which includes many useful plugins for biomedical image analysis. Some Fiji macros are provided in the Tools page. Please feel free to reach us for explanation or for help to write a macro for your needs.